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KMID : 0358319960370121333
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Korean Journal of Urology
1996 Volume.37 No. 12 p.1333 ~ p.1338
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Effect of Tumor Necrosis Factor-alpha Gene Transduced Bladder Tumor Cell Xenograft on Survival of C3H/He Mice
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Abstract
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Objective:
@EN To evaluate the effect of tumor necrosis factor-α (TNF-α) gene transduced bladder tumor cells on survival of C3H/He mice.
@ES Materials and Methods:
@EN The complete cDNA of TNF-α was introduced to mouse bladder tumor cell line(MBT-2) using a retroviral vector. Gene transfer and expression were confirmed by polymerase chain reactijon9PCR0 and reverse transcription polymerase chain
reaction9RT-PCR).
Produced TNF-α was measured by ELISA. TNF-α gene transduced and parental cells were xenografted in C3H/He mice. Implantation and growth rate and survial of experimental animals were analysed.
@ES Results:
@EN Successful gene transfer and expression were confirmed. TNF-α gene transduced MBT-2 cells secreted TNF-α at the concentration of 47.4pg/10E6cells/24hours. The implantation and growth rate of TNF-α gene transduced MBT-2 cells much lower
than
those
of parental cells and survival of experimental animals was different between TNF-α gene transduced MBT-2 and parental cells(P<0.05).
@ES conclusion:
@EN This study shows potential advantages of localized TNF-α secretion to induce potent antitumorigenic response. Tumor cell targeted TNF-α gene therapy could become a useful tool in treatment of bladder cancer.
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KEYWORD
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